Journal: Biomolecular Detection and Quantification

Article Title: Three-color crystal digital PCR

doi: 10.1016/j.bdq.2016.10.002

Figure Lengend Snippet: Crystal Digital PCR Workflow. A. The samples are pipetted into the Sapphire chip, which is then sealed using caps. B. The chips are then placed onto the Naica Geode, and the program that will generate droplets prior to thermal cycling to endpoint is started by the user. C. When thermocycling is completed, the chips are transferred to the Naica Prism3, and images acquired for the Blue, Green and Red detection channels. D. Finally, data is analyzed using the Crystal Miner software, which integrates quality control instead of your experiment, post-treatment such as fluorescence spillover compensation, as well as the calculation of concentrations.

Article Snippet: The Naica Prism3 (Stilla Technologies) is an automated fluorescence microscope comprising a CCD camera equipped with a tri-band emission filter (495–520 nm//560–610 nm//655–720 nm), a motorized x-y-z stage for sample positioning and 3 sets of independently actionable high power LEDs with band pass filters of 415–480 nm (blue channel), 530–550 nm (green channel) and 615–645 nm (red channel) for fluorescence excitation.

Techniques: Digital PCR, Software, Control, Fluorescence

Journal: Biomolecular Detection and Quantification

Article Title: Three-color crystal digital PCR

doi: 10.1016/j.bdq.2016.10.002

Figure Lengend Snippet: Naica Prism3 detection channels specifications.

Article Snippet: The Naica Prism3 (Stilla Technologies) is an automated fluorescence microscope comprising a CCD camera equipped with a tri-band emission filter (495–520 nm//560–610 nm//655–720 nm), a motorized x-y-z stage for sample positioning and 3 sets of independently actionable high power LEDs with band pass filters of 415–480 nm (blue channel), 530–550 nm (green channel) and 615–645 nm (red channel) for fluorescence excitation.

Techniques:

Journal: Biomolecular Detection and Quantification

Article Title: Three-color crystal digital PCR

doi: 10.1016/j.bdq.2016.10.002

Figure Lengend Snippet: Visualization of droplet crystals. Droplet Crystal containing 29078 analyzable droplets, imaged post PCR using the Blue (A), Green (B) and Red (C) Prism3 acquisition channels. The left insert in A. presents a zoomed-in portion of the droplet crystal. Droplets in which no amplification has taken place remain dark, and droplets in which amplification is observed are lighter in color. Droplets have a higher fluorescent baseline in the Blue channel due to the FITC added to the reaction mix.

Article Snippet: The Naica Prism3 (Stilla Technologies) is an automated fluorescence microscope comprising a CCD camera equipped with a tri-band emission filter (495–520 nm//560–610 nm//655–720 nm), a motorized x-y-z stage for sample positioning and 3 sets of independently actionable high power LEDs with band pass filters of 415–480 nm (blue channel), 530–550 nm (green channel) and 615–645 nm (red channel) for fluorescence excitation.

Techniques: Amplification

Journal: Biomolecular Detection and Quantification

Article Title: Three-color crystal digital PCR

doi: 10.1016/j.bdq.2016.10.002

Figure Lengend Snippet: Light path for fluorescence excitation and emission within the Naica Prism3. Samples (Droplets) are illuminated sequentially by the Blue, Green and Red LED, and re-emit light in the form of fluorescence. Light emitted passes through tri-band filter to generate the resulting image for the corresponding channel.

Article Snippet: The Naica Prism3 (Stilla Technologies) is an automated fluorescence microscope comprising a CCD camera equipped with a tri-band emission filter (495–520 nm//560–610 nm//655–720 nm), a motorized x-y-z stage for sample positioning and 3 sets of independently actionable high power LEDs with band pass filters of 415–480 nm (blue channel), 530–550 nm (green channel) and 615–645 nm (red channel) for fluorescence excitation.

Techniques: Fluorescence

Journal: Biomolecular Detection and Quantification

Article Title: Three-color crystal digital PCR

doi: 10.1016/j.bdq.2016.10.002

Figure Lengend Snippet: Spectral overlap for FAM and Yakima Yellow (YY) fluorophores and its detection using the Prism3 device. On each graph, wavelength (nm) is plotted on the x axis, and normalized signal intensity on the y axis. A. Both FAM and Yakima Yellow contribute to the image for the Blue detection channel. B. The image for the Green detection channel is based solely on the signal from the Yakima Yellow fluorophore. C. Neither FAM, nor Yakima Yellow are excited by the light emitted by the Red LED, there is no signal for the Red detection channel.

Article Snippet: The Naica Prism3 (Stilla Technologies) is an automated fluorescence microscope comprising a CCD camera equipped with a tri-band emission filter (495–520 nm//560–610 nm//655–720 nm), a motorized x-y-z stage for sample positioning and 3 sets of independently actionable high power LEDs with band pass filters of 415–480 nm (blue channel), 530–550 nm (green channel) and 615–645 nm (red channel) for fluorescence excitation.

Techniques:

Journal: Biomolecular Detection and Quantification

Article Title: Three-color crystal digital PCR

doi: 10.1016/j.bdq.2016.10.002

Figure Lengend Snippet: Spectral overlap for FAM, Yakima Yellow and Cy5 fluorophores and detection using the Prism3 device. On each graph, wavelength (nm) is plotted on the x axis, and normalized signal intensity on the y axis. A. Both FAM and Yakima Yellow contribute to the image for the Blue Detection Channel. B. Both Yakima Yellow and Cy5 contribute to the image for the Green Detection Channel. C. Only Cy5 contributes to the image for the Red Detection Channel.

Article Snippet: The Naica Prism3 (Stilla Technologies) is an automated fluorescence microscope comprising a CCD camera equipped with a tri-band emission filter (495–520 nm//560–610 nm//655–720 nm), a motorized x-y-z stage for sample positioning and 3 sets of independently actionable high power LEDs with band pass filters of 415–480 nm (blue channel), 530–550 nm (green channel) and 615–645 nm (red channel) for fluorescence excitation.

Techniques: